محققین نے خلیوں میں مخصوص پروٹینوں کا پتہ لگانے کے لیے نئے اور زیادہ موثر جینیاتی رپورٹر پروٹین بنائے ہیں ، ایک ایسا مرحلہ جس کے بارے میں خیال کیا جاتا ہے کہ جینیاتی مواد اور انٹرا سیلولر عمل کے مطالعہ میں مدد ملے گی ، انجینئرڈ یا دوسری صورت میں۔ جینیاتی طور پر انکوڈ شدہ رپورٹر پروٹین بائیو ٹیکنالوجی کے شعبوں میں بہت زیادہ استعمال ہوتے ہیں۔ یہ رپورٹر پروٹین بعض پروٹینوں کا پتہ لگانے میں مدد کرتے ہیں اور انجینئرڈ جینیاتی سرکٹس کو ڈی کوڈ کرتے ہیں۔ تاہم ، روایتی رپورٹر پروٹین پروٹین انووں کے فلوروسینس پر انحصار کرتے ہیں ، جس کی وجہ سے کچھ تناؤ کا پتہ لگانا مشکل ہوجاتا ہے۔ تاہم ، واشنگٹن یونیورسٹی اور مائیکروسافٹ کے محققین نے رپورٹر پروٹین تیار کیے ہیں جنہیں ‘نانو پور سینسنگ ڈیوائس’ کے ذریعے پڑھا جا سکتا ہے۔
محققین نے اس نئے رپورٹر پروٹین کو ‘نینو پور ایڈریس ایبل پروٹین ٹیگز انجینئرڈ بطور رپورٹر’ (نانو پورٹیرز ، یا این ٹی ای آر) کہا ہے۔ ٹیم نے ایسے 20 این ٹی ای آر ٹیگ تیار کیے ہیں اور انہیں لائبریری میں محفوظ کیا ہے۔
تحقیقی رپورٹ نیچر بائیو ٹیکنالوجی میں شائع ہوئی۔ رپورٹر پروٹین محققین کو سیل کے عمل اور بے ضابطگیوں کے بارے میں ڈیٹا اکٹھا کرنے میں مدد دے سکتے ہیں۔ روایتی طور پر ، صرف آپٹیکل پروٹین جنہوں نے فلوروسینٹ اثرات دکھائے تھے ، آزمائشی اور غلطی کے طریقہ کار کے ذریعے پتہ لگایا جا سکتا ہے۔ زیادہ سے زیادہ پروٹین اسٹرینڈز جن کا بیک وقت مطالعہ کیا جا سکتا تھا وہ بھی محدود تھے۔ یہ بڑی حد تک محدود سیلولر سطح کی تحقیق ہے۔
تاہم ، نئے مصنوعی پروٹین سیل کے ماحول کے بارے میں معلومات جمع کرنے کے لیے سیل کے باہر خفیہ ہوتے ہیں۔ وہ الگ الگ امینو ایسڈ “بارکوڈ” رکھتے ہیں جو نانو پور ڈٹیکٹر کو جواب دیتے ہیں۔ مطالعہ کے لیے ، محققین نے آکسفورڈ نینو پور ٹیکنالوجیز منین ڈیوائس کا استعمال کیا۔ ان رپورٹر پروٹینوں کے ساتھ ، یہ بھی ممکن ہے کہ بیک وقت مزید پروٹین اسٹرینڈ پڑھیں ، جو کم از کم 10 گنا زیادہ ملٹی پلیکسنگ کے مواقع فراہم کرتے ہیں۔
این ٹی ای آر پروٹین ہیں جن پر چارج شدہ “دم” ہوتے ہیں جو انہیں برقی میدان کے ذریعے نانو پور کے سینسرز کی طرف راغب کرتے ہیں۔ محققین ، پھر ان برقی سگنلز کو ڈی کوڈ کرنے اور انہیں NTER بارکوڈز میں درجہ بندی کرنے کے لیے مشین لرننگ کا استعمال کرتے ہیں۔
“یہ خلیوں اور کمپیوٹرز کے درمیان بنیادی طور پر نیا انٹرفیس ہے ،” یوریک الرٹ کی ایک رپورٹ نے نو مصنفین میں سے ایک اور واشنگٹن ریسرچ کے اسسٹنٹ پروفیسر جیف نیوالا کے حوالے سے کہا۔
لیڈ شریک مصنف کیرن ژانگ نے ان این ٹی ای آر کو 20 ٹیگز سے آگے بڑھانے کی صلاحیت دیکھی۔ انہوں نے اسی رپورٹ میں کہا ، “ہم فی الحال نانو پورٹیرز کی تعداد کو سینکڑوں ، ہزاروں ، شاید لاکھوں تک بڑھانے کے لیے کام کر رہے ہیں۔”
NTERs بیماریوں کا پتہ لگانے کے طریقے کو تبدیل کر سکتے ہیں یا جسم کے مخصوص علاقوں میں علاج معالجے کو نشانہ بناتے ہیں۔ اور “پیچیدہ جینیاتی سرکٹ ڈیزائن کو ڈیبگ کرنا” ایک اور فیلڈ ہے جو اس تحقیق سے فائدہ اٹھائے گا۔
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